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behavior 171 2025 105726 virions  (Addgene inc)


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    Structured Review

    Addgene inc behavior 171 2025 105726 virions
    Behavior 171 2025 105726 Virions, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a30p+plasmids/pT7-7+asyn+A30P+(Plasmid+%23105726)/pm40153918-72-14-31
    Average 93 stars, based on 1 article reviews
    behavior 171 2025 105726 virions - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Temperature is a key determinant of alpha- and beta-synuclein membrane interactions in neurons
    Article Snippet: .. 293-T cells were transfected with αS wt or A30P plasmids along with pMD2.G and psPAX2 (packaging plasmids: Addgene #12259 and #12260, respectively). ..

    Article Title: Temperature is a key determinant of alpha- and beta-synuclein membrane interactions in neurons
    Article Snippet: .. 293-T cells were transfected with αS wt or A30P plasmids along with pMD2.G and psPAX2 (packaging plasmids: Addgene #12259 and #12260, respectively). ..



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    The human αS <t>A30P</t> mutant is predominantly cytosolic. Cytosolic and membrane fractions were sequentially extracted from cortical neurons expressing human wt or A30P αS at 37 °C ( A ). The solubility of control proteins GAPDH and calnexin is depicted in panel B . Representative western blot images to the quantification are shown in panel C . Digitonin concentration, 800 or 900 μg/ml. (C, cytosol and M, membrane). N = 3 independent experiments, performed on different days in n = 12 + 4 + 4 independent wells (total n = 20). ∗∗∗∗ p < 0.0001. ns, not significant.
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    The human αS <t>A30P</t> mutant is predominantly cytosolic. Cytosolic and membrane fractions were sequentially extracted from cortical neurons expressing human wt or A30P αS at 37 °C ( A ). The solubility of control proteins GAPDH and calnexin is depicted in panel B . Representative western blot images to the quantification are shown in panel C . Digitonin concentration, 800 or 900 μg/ml. (C, cytosol and M, membrane). N = 3 independent experiments, performed on different days in n = 12 + 4 + 4 independent wells (total n = 20). ∗∗∗∗ p < 0.0001. ns, not significant.
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    Image Search Results


    The human αS A30P mutant is predominantly cytosolic. Cytosolic and membrane fractions were sequentially extracted from cortical neurons expressing human wt or A30P αS at 37 °C ( A ). The solubility of control proteins GAPDH and calnexin is depicted in panel B . Representative western blot images to the quantification are shown in panel C . Digitonin concentration, 800 or 900 μg/ml. (C, cytosol and M, membrane). N = 3 independent experiments, performed on different days in n = 12 + 4 + 4 independent wells (total n = 20). ∗∗∗∗ p < 0.0001. ns, not significant.

    Journal: The Journal of Biological Chemistry

    Article Title: Temperature is a key determinant of alpha- and beta-synuclein membrane interactions in neurons

    doi: 10.1016/j.jbc.2021.100271

    Figure Lengend Snippet: The human αS A30P mutant is predominantly cytosolic. Cytosolic and membrane fractions were sequentially extracted from cortical neurons expressing human wt or A30P αS at 37 °C ( A ). The solubility of control proteins GAPDH and calnexin is depicted in panel B . Representative western blot images to the quantification are shown in panel C . Digitonin concentration, 800 or 900 μg/ml. (C, cytosol and M, membrane). N = 3 independent experiments, performed on different days in n = 12 + 4 + 4 independent wells (total n = 20). ∗∗∗∗ p < 0.0001. ns, not significant.

    Article Snippet: 293-T cells were transfected with αS wt or A30P plasmids along with pMD2.G and psPAX2 (packaging plasmids: Addgene #12259 and #12260, respectively).

    Techniques: Mutagenesis, Membrane, Expressing, Solubility, Control, Western Blot, Concentration Assay